PSV-B-14 Bovine Luteal Cell Apoptosis Following Administration of a Commercial Inactivated or Modified-Live Virus Vaccine at Time of AI
نویسندگان
چکیده
Abstract Damage to the bovine corpus luteum (CL) has been reported following modified-live virus vaccination (MLV). The objective of this study was investigate degree luteal apoptosis after MLV or inactivated vaccine (IV) administration at time artificial insemination. Beef females were estrous synchronized and on d 0 vaccinated with a IV being transrectally ultrasounded record presence location dominant follicles CL. Thirteen cows (MLV, n=7; IV, n=6) selected 10-13 across treatment based follicle size estrus expression for ovariectomy. Ovaries obtained CL frozen in OCT sectioned immunohistochemistry analysis. At ovary collection, some animals had two These characterized into old new CL, relative if ovulation occurred response synchronization protocol afterward due an abnormal cycle, respectively. For analysis, nonconsecutive sections evaluated using TUNEL staining kit (ab66110) according manufacturer’s instructions, modification DAPI used as cell marker. Two random fields view from each section photographed Keyence BZ-X810 microscope number total apoptotic cells. GLIMMIX procedure SAS utilized analyze percentage cells image included effect. Percentage affected by (P < 0.0001). MLV-new greatest (35.56 ± 5.5%) apoptosis, while MLV-old (7.05 4.6%) (4.24 4.9%) similarly decreased. In summary, greater which formed cycle indicates ability induce dysfunction extend its effects development function.
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ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 2022
ISSN: ['0021-8812', '1525-3163', '1525-3015', '1544-7847']
DOI: https://doi.org/10.1093/jas/skac247.631